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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
25/03/2024 |
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Data da última atualização: |
25/03/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOARES, F. M.; PIRES, L. F.; GARCIA, M. C.; CORADIN, L.; GHILARDI-LOPES, N. P.; SILVA, R. R.; CARVALHO, A. M. de; GAVAI, A.; BOUZEMBRAK, Y.; MACULAN, B. C. M. dos S.; KOFFLER, S.; MONTEDO, U. B.; DRUCKER, D. P.; SANTIAGO, R.; CARVALHO, M. C. P. de; LIMA, A. C. da S.; GABRIEL, H. D. E.; FRANÇA, S. G. M. de; ALMEIDA, K. R. de; SANTOS, B. J. dos; SARAIVA, A. M. |
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Afiliação: |
FILIPI MIRANDA SOARES, UNIVERSIDADE DE SÃO PAULO, UNIVERSITY OF TWENTE; LUÍS FERREIRA PIRES, UNIVERSITY OF TWENTE; MARIA CAROLINA GARCIA, CENTRO UNIVERSITÁRIO BELAS ARTES DE SÃO PAULO; LIDIO CORADIN, PLANTS FOR THE FUTURE PROJECT; NATALIA PIRANI GHILARDI-LOPES, UNIVERSIDADE FEDERAL DO ABC; RUBENS RANGEL SILVA, CENTRO UNIVERSITÁRIO UNA; ALINE MARTINS DE CARVALHO, UNIVERSIDADE DE SÃO PAULO; ANAND GAVAI, UNIVERSITY OF TWENTE; YAMINE BOUZEMBRAK, WAGENINGEN UNIVERSITY AND RESEARCH; BENILDES COURA MOREIRA DOS SANTOS MACULAN, UNIVERSIDADE FEDERAL DE MINAS GERAIS; SHEINA KOFFLER, UNIVERSIDADE DE SÃO PAULO; UIARA BANDINELI MONTEDO, UNIVERSIDADE DE SÃO PAULO; DEBORA PIGNATARI DRUCKER, CNPTIA; RAQUEL SANTIAGO, UNIVERSIDADE FEDERAL DE GOIÁS; MARIA CLARA PERES DE CARVALHO, UNIVERSIDADE DE SÃO PAULO; ANA CAROLINA DA SILVA LIMA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; HILLARY DANDARA ELIAS GABRIEL, UNIVERSIDADE DE SÃO PAULO; STEPHANIE GABRIELE MENDONÇA DE FRANÇA, UNIVERSIDADE DE SÃO PAULO; KAROLINE REIS DE ALMEIDA, UNIVERSIDADE DE SÃO PAULO; BÁRBARA JUNQUEIRA DOS SANTOS, UNIVERSIDADE DE SÃO PAULO; ANTONIO MAURO SARAIVA, UNIVERSIDADE DE SÃO PAULO. |
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Título: |
Citizen science data on urban forageable plants: a case study in Brazil. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Gigabyte, v. 2024, p. 1-16, Feb. 2024. |
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ISSN: |
2709-4715 |
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DOI: |
10.46471/gigabyte.107 |
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Idioma: |
Inglês |
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Conteúdo: |
This paper presents two key data sets derived from the Pomar Urbano project. The first data set is a comprehensive catalog of edible fruit-bearing plant species, native or introduced to Brazil. The second data set, sourced from the iNaturalist platform, tracks the distribution and monitoring of these plants within urban landscapes across Brazil. The study includes data from the capitals of all 27 federative units of Brazil, focusing on the ten cities that contributed the most observations as of August 2023. The research emphasizes the significance of citizen science in urban biodiversity monitoring and its potential to contribute to various fields, including food and nutrition, creative industry, study of plant phenology, and machine learning applications. |
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Palavras-Chave: |
Áreas urbanas; Biodiversidade urbana; Ciência cidadã; Ciência vegetal; Citizen science; Plantas forrageiras. |
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Thesagro: |
Botânica; Ciência Animal; Ecologia. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1163118/1/AP-Citizen-science-2024.pdf
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Marc: |
LEADER 02127naa a2200493 a 4500
001 2163118
005 2024-03-25
008 2024 bl uuuu u00u1 u #d
022 $a2709-4715
024 7 $a10.46471/gigabyte.107$2DOI
100 1 $aSOARES, F. M.
245 $aCitizen science data on urban forageable plants$ba case study in Brazil.$h[electronic resource]
260 $c2024
520 $aThis paper presents two key data sets derived from the Pomar Urbano project. The first data set is a comprehensive catalog of edible fruit-bearing plant species, native or introduced to Brazil. The second data set, sourced from the iNaturalist platform, tracks the distribution and monitoring of these plants within urban landscapes across Brazil. The study includes data from the capitals of all 27 federative units of Brazil, focusing on the ten cities that contributed the most observations as of August 2023. The research emphasizes the significance of citizen science in urban biodiversity monitoring and its potential to contribute to various fields, including food and nutrition, creative industry, study of plant phenology, and machine learning applications.
650 $aBotânica
650 $aCiência Animal
650 $aEcologia
653 $aÁreas urbanas
653 $aBiodiversidade urbana
653 $aCiência cidadã
653 $aCiência vegetal
653 $aCitizen science
653 $aPlantas forrageiras
700 1 $aPIRES, L. F.
700 1 $aGARCIA, M. C.
700 1 $aCORADIN, L.
700 1 $aGHILARDI-LOPES, N. P.
700 1 $aSILVA, R. R.
700 1 $aCARVALHO, A. M. de
700 1 $aGAVAI, A.
700 1 $aBOUZEMBRAK, Y.
700 1 $aMACULAN, B. C. M. dos S.
700 1 $aKOFFLER, S.
700 1 $aMONTEDO, U. B.
700 1 $aDRUCKER, D. P.
700 1 $aSANTIAGO, R.
700 1 $aCARVALHO, M. C. P. de
700 1 $aLIMA, A. C. da S.
700 1 $aGABRIEL, H. D. E.
700 1 $aFRANÇA, S. G. M. de
700 1 $aALMEIDA, K. R. de
700 1 $aSANTOS, B. J. dos
700 1 $aSARAIVA, A. M.
773 $tGigabyte$gv. 2024, p. 1-16, Feb. 2024.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
27/01/2021 |
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Data da última atualização: |
27/01/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUTO, B. de M.; ARAÚJO, A. C. B. de; HAMANN, P. R. V.; BASTOS, A. de R.; CUNHA, I. de S.; PEIXOTO, J.; KRUGER, R. H.; NORONHA, E. F.; QUIRINO, B. F. |
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Afiliação: |
BETULIA DE MORAIS SOUTO, CNPAE; Ana Carolina Bitencourt de Araújo; Pedro Ricardo Vieira Hamann, Universidade de Brasília; Andrêssa de Rezende Bastos; Isabel de Souza Cunha, Universidade Católica de Brasília; Julianna Peixoto, Universidade de Brasília; Ricardo Henrique Kruger, Universidade de Brasília; Eliane Ferreira Noronha, Universidade de Brasília; BETANIA FERRAZ QUIRINO, CNPAE. |
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Título: |
Functional screening of a Caatinga goat (Capra hircus) rumen metagenomic library reveals a novel GH3 beta-xylosidase. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
PLoS ONE, v. 16, n. 1, e024511, 2021. |
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DOI: |
https://doi.org/10.1371/journal.pone.0245118 |
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Idioma: |
Inglês |
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Conteúdo: |
Functional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized. Recombinant BGL11 was obtained and kinetically characterized. Substrate specificity of the purified protein was assessed using seven synthetic aryl substrates. Activity towards nitrophenyl-beta-D-glucopyranoside (pNPG), 4-nitrophenyl- beta -D-xylopyranoside (pNPX) and 4-nitrophenyl- beta -D-cellobioside (pNPC) suggested that BGL11 is a multifunctional enzyme with beta-glucosidase, beta-xylosidase, and cellobiohydrolase activities. However, further testing with five natural substrates revealed that, although BGL11 has multiple substrate specificity, it is most active towards xylobiose. Thus, in its native goat rumen environment, BGL11 most likely functions as an extracellular beta-xylosidase acting on hemicellulose. Biochemical characterization of BGL11 showed an optimal pH of 5.6, and an optimal temperature of 50°C. Enzyme stability, an important parameter for industrial application, was also investigated. At 40°C purified BGL11 remained active for more than 15 hours without reduction in activity, and at 50°C, after 7 hours of incubation, BGL11 remained 60% active. In contrast to BLG11, most beta-xylosidases kinetically studied belong to the GH43 family and have been characterized only using synthetic substrates. In industry, beta-xylosidases can be used for plant biomass deconstruction, and the released sugars can be fermented into valuable bio-products, ranging from the biofuel ethanol to the sugar substitute xylitol. MenosFunctional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized. Recombinant BGL11 was obtained and kinetically characterized. Substrate specificity of the purified protein was assessed using seven synthetic aryl substrates. Activity towards nitrophenyl-beta-D-glucopyranoside (pNPG), 4-nitrophenyl- beta -D-xylopyranoside (pNPX) and 4-nitrophenyl- beta -D-cellobioside (pNPC) suggested that BGL11 is a multifunctional enzyme with beta-glucosidase, beta-xylosidase, and cellobiohydrolase activities. However, further testing with five natural substrates revealed that, although BGL11 has multiple substrate specificity, it is most active towards xylobiose. Thus, in its native goat rumen environment, BGL11 most likely functions as an extracellular beta-xylosidase acting on hemicellulose. Biochemical characterization of BGL11 showed an optimal pH of 5.6, and an optimal temperature of 50°C. Enzyme stability, an important parameter for industrial application, was also investigated. At 40°C purified BGL11 remained active for more than 15 hours without reduction in activity, and at 50°C, after 7 hours of incubation, BGL11 remained 60% active. In contrast to BLG11, most beta-xylosidases kinetical... Mostrar Tudo |
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Thesagro: |
Clonagem; Enzima; Peptídeo; Programa de Computador. |
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Thesaurus NAL: |
Cloning (animals); Computer software; Enzymes; Metagenomics; Prevotella; Screening; Signal peptide. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/220708/1/Functional-screening-of-a-Caatinga-goat-2021.pdf
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Marc: |
LEADER 02852naa a2200361 a 4500
001 2129624
005 2021-01-27
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0245118$2DOI
100 1 $aSOUTO, B. de M.
245 $aFunctional screening of a Caatinga goat (Capra hircus) rumen metagenomic library reveals a novel GH3 beta-xylosidase.$h[electronic resource]
260 $c2021
520 $aFunctional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized. Recombinant BGL11 was obtained and kinetically characterized. Substrate specificity of the purified protein was assessed using seven synthetic aryl substrates. Activity towards nitrophenyl-beta-D-glucopyranoside (pNPG), 4-nitrophenyl- beta -D-xylopyranoside (pNPX) and 4-nitrophenyl- beta -D-cellobioside (pNPC) suggested that BGL11 is a multifunctional enzyme with beta-glucosidase, beta-xylosidase, and cellobiohydrolase activities. However, further testing with five natural substrates revealed that, although BGL11 has multiple substrate specificity, it is most active towards xylobiose. Thus, in its native goat rumen environment, BGL11 most likely functions as an extracellular beta-xylosidase acting on hemicellulose. Biochemical characterization of BGL11 showed an optimal pH of 5.6, and an optimal temperature of 50°C. Enzyme stability, an important parameter for industrial application, was also investigated. At 40°C purified BGL11 remained active for more than 15 hours without reduction in activity, and at 50°C, after 7 hours of incubation, BGL11 remained 60% active. In contrast to BLG11, most beta-xylosidases kinetically studied belong to the GH43 family and have been characterized only using synthetic substrates. In industry, beta-xylosidases can be used for plant biomass deconstruction, and the released sugars can be fermented into valuable bio-products, ranging from the biofuel ethanol to the sugar substitute xylitol.
650 $aCloning (animals)
650 $aComputer software
650 $aEnzymes
650 $aMetagenomics
650 $aPrevotella
650 $aScreening
650 $aSignal peptide
650 $aClonagem
650 $aEnzima
650 $aPeptídeo
650 $aPrograma de Computador
700 1 $aARAÚJO, A. C. B. de
700 1 $aHAMANN, P. R. V.
700 1 $aBASTOS, A. de R.
700 1 $aCUNHA, I. de S.
700 1 $aPEIXOTO, J.
700 1 $aKRUGER, R. H.
700 1 $aNORONHA, E. F.
700 1 $aQUIRINO, B. F.
773 $tPLoS ONE$gv. 16, n. 1, e024511, 2021.
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Embrapa Agroenergia (CNPAE) |
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